Influence of Wnt-1 recombinant adenovirus on differentiation of human epidermal stem cells / 中华烧伤杂志

<p><b>OBJECTIVE</b>To observe the influence of Wnt-1 recombinant adenovirus on differentiation tendency of human epidermal stem cells.</p><p><b>METHODS</b>Wnt-1 recombinant adenovirus was transduced into hESCs (E group), while normal hESCs were used as control (C) group. The diameter, proliferation,and labeling molecular expression of hESC were determined. The content of MMP-2 and MMP-7 in supernate were also assayed.</p><p><b>RESULTS</b>There was no obvious difference in diameter of hESC between two groups. The density of hESC in E group was (1.45 +/- 0.09) x 10(5)/mL, which was obviously higher than that in C group [(1.18 +/- 0.10) x 10(5)/mL, P < 0.05]. There were no obvious differences in expression of markers between two groups,including keratin 5 (KS), K6, K7, KS, K14, CD44, carcinoembryonic-like antigen (CEAA), ER, PR (P > 0.05) ,while the expression of K 10 was different among groups [(60 +/- 3)% in E group, 0 in C group], also K18 [(34.3 +/- 2.1)% in E group vs. (13.8 +/- 1.7)% in C group, P < 0.05], and K19 [(17.1 +/- 1.8)% in E group vs. (24.4 +/- 1.5)% in C group, P < 0.05].The contents of MMP-2 and MMP-7 in E group were higher than those in C group (P < 0.01).</p><p><b>CONCLUSION</b>Wnt-1 recombinant adenovirus can induce the differentiation of hESCs to glandular epithelium-like cells.</p>

The study of treatment of osteoporotic thoracolumbar multi-vertebral compressive fractures by kyphoplasty with one balloon / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the effect of kyphoplasty in treating osteoporotic thoracolumbar multi-vertebral compressive fractures with one balloon.</p><p><b>METHODS</b>thirteen cases (37 vertebral bodies) of osteoporotic thoracolumbar multi-vertebral compressive fractures were treated. The patients were women, range 65 to 79 years old, and all with well intact posterior wall of vertebral body. 37 thoracolumbar vertebral bodies were treated by Kyphoplasty with one balloon under "C" armed imaging guiding.</p><p><b>RESULTS</b>All 13 patients tolerated well. The mean height of the anterior, media and posterior vertebral bodies was (1.9 +/- 0.5) cm, (1.5 +/- 0.5) cm, (2.6 +/- 0.5) cm preoperatively and (2.1 +/- 0.4) cm, (2.2 +/- 0.4) cm, (2.8 +/- 0.5) cm postoperatively (P < 0.001). The mean kyphosis was improved from (31.3 +/- 14.1) degrees to (24.8 +/- 11.3) degrees. There was not back pain and deformed correction was maintained well after 17.3 months' follow up, No complication occurred.</p><p><b>CONCLUSION</b>The balloon was not damaged, because the pressure was lower than usual pressure, the cost of patients was saved. In this study, we fulfilled small amount of cement in vertebral body which reduced the leakage of cement. Fracture of close vertebral body was also reduced because of lower stiffness and modulus of elasticity of adjacent vertebral body.</p>